| Product name | Human TGF-β3 protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Human TGF-β3 protein (Ala301-Ser412) was expressed with 6×His tag at the C-terminus |
| Applications | His tag |
| Applications notes | Human |
| Activity | Measure by its ability to inhibit IL-4-induce proliferation in HT-2 cells.The ED₅₀ for this effect is <50 pg/mL.The specific activity of recombinant human TGF beta 3 is > 2 x 10⁷ IU/mg. |
| Protein length | The recombinant Human TGF-β3 consists of 112 amino acids and predicts a molecular mass of 13.66 kDa |
| Preparation method | E. coli |
| Purity | >98% as determined by SDS-PAGE. |
| Alternative | ARVD, ARVD1, LDS5, RNHF, TGFB3, TGF-B3 |
| Formulation | The protein was lyophilized from 0.01% sarkosyl in 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us. |
| Features & Benefits | Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | The protein has a calculated MW of 13.66 kDa. The protein migrates as 13 kDa under reducing condition (SDS-PAGE analysis). |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Human TGF-β3 protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein ( (5% HSA , 10%FBS or 0.1%BSA)). Please prevent freeze-thaw cycles |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Transforming Growth Factors-Beta 3 (TGF-beta 3) is belonging to the TGF-β superfamily TGF-β3 is 86%, 91% similar to TGF-β1 and TGF-β2. TGF-beta 3 is a 12.8 kDa protein containing 412 amino acids, which could inhibitor of DNA synthesis, increases cellular proliferation, essential mediator of EMT in cardiac morphogenesis, and up-regulated by milk stasis and induces apoptosis in mammary gland epithelium during involution. |
| Alternative | ARVD, ARVD1, LDS5, RNHF, TGFB3, TGF-B3 |
| Accession | P10⁶00 |
Fig.SDS-PAGE analysis of Human TGF-β3 protein
Author：W Yan, M Shen, K Sun, S Li, J Miao, J Wang, J Xu Publication name：Biomedicines IF：4.7
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