| Product name | Human RANKL Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Human RANKL (Ala142-Asp317)(O14788) was expressed with 6×His tag at the C-terminus |
| Applications | His tag |
| Applications notes | Human |
| Activity | Measure by its ability to induce osteoclast differentiation in RAW264.7 cells.The ED₅₀ for this effect is <10 ng/mL. |
| Protein length | The recombinant Human RANKL consists of 176 amino acids and predicts a molecular mass of 19.9 kDa |
| Preparation method | E. coli |
| Purity | >98% as determined by SDS-PAGE. |
| Alternative | soluble Receptor Activator of NF-kB Ligand, TNFSF11, TRANCE (TNF-Related Activation-induced Cytokine), OPGL, ODF (Osteoclast Differentiation Factor), CD254,sRNAK Ligand |
| Formulation | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us. |
| Features & Benefits | Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | The protein has a calculated MW of 20.67 kDa. The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis). |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Human RANKL Protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions | Lyophilized protein protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4 °C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Tumor necrosis factor ligand superfamily member 11, also known as receptor activator of nuclear factor kappa-B ligand, Osteoprotegerin ligand, TNFSF11, RANKL, TRANCE, OPGL and CD254, is a single-pass type II membrane protein that belongs to the tumor necrosis factor family. The receptor activator of nuclear factor-kappa B ligand (RANKL), its cognate receptor RANK, and its natural decoy receptor osteoprotegerin have been identified as the final effector molecules of osteoclastic bone resorption. RANK and RANKL are key regulators of bone remodeling and regulate T cell/dendritic cell communications, and lymph node formation. Moreover, RANKL and RANK are expressed in mammary gland epithelial cells and control the development of a lactating mammary gland during pregnancy |
| Alternative | soluble Receptor Activator of NF-kB Ligand, TNFSF11, TRANCE (TNF-Related Activation-induced Cytokine), OPGL, ODF (Osteoclast Differentiation Factor), CD254,sRNAK Ligand |
| Accession | O14788 |
Fig.SDS-PAGE analysis of Huamn RANKL protein
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