| Product name | Human/Mouse/Rat BDNF protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Human/Mouse/Rat BDNF protein (His129-Arg247) with 6×His tag at the C-terminus was expressed |
| Applications | His tag |
| Applications notes | Human/Mouse/Rat |
| Activity | Measure by its ability to induce proliferation in BaF3 cells transfected with TrkB.The ED₅₀ for this effect is <2 ng/mL. |
| Protein length | The recombinant Human/Mouse/Rat BDNF protein consists of 119 amino acids and predicts a molecular mass of 14.45 kDa |
| Preparation method | E. coli |
| Purity | >98% as determined by SDS-PAGE. |
| Alternative | ANON2, BULN2 |
| Formulation | The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5. If you have any concerns or special requirements, please confirm with us. |
| Features & Benefits | Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | The protein has a calculated MW of 14.45 kDa. The protein migrates as 14 kDa under reducing condition (SDS-PAGE analysis). |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Human/Mouse/Rat BDNF protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Brain Derived Neurotrophic Factor (BDNF) is a neurotrophin that belongs to NGF-beta family. BDNF can bind to its high affinity receptor TrkB and activates signal transduction cascades (IRS1/2, PI3K, Akt). BDNF can also bind to the p75NTR, but the affinity for the p75NTR receptor is lower than for TrkB. BDNF is a neurotransmitter modulator, and is vital in maturation, survival and differentiation of neuronal populations during development. BDNF also participates in neuronal plasticity, which is essential for learning and memory. BDNF is widely expressed in the CNS. |
| Alternative | ANON2, BULN2 |
| Accession | P23560(Human), P21237(Mouse), P23363(Rat) Q9510⁶ |
Fig.SDS-PAGE analysis of Human/Mouse/Rat BDNF protein
Author：Priyadharshini, K. I., Balakrishnan, S., Saranyan, R., Nirmal, M., & Murugesan, A Publication name：Journal of Cancer Research and Therapeutics IF：1.3
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