| Product name |
Human/Mouse/Rat Activin A Protein (Animal-Free) |
| Sequence |
Amino acid sequence derived from Human/Mouse/Rat Activin A Protein (Gly311-Ser426) (Human: P08476, Mouse: Q04998, Rat: P18331) was expressed. |
| Applications |
No tag |
| Applications notes |
Human |
| Activity |
Measure by its ability to inhibit the proliferation of mouse MPC-11 cells. The ED₅₀ for this effect is <10 ng/mL. The specific activity of recombinant human Activin A is approximately >1.0 x 10³ IU/mg. |
| Protein length |
The recombinant Human/Mouse/Rat Activin A Protein consists of 116 amino acids and predicts a molecular mass of 13.1 kDa. |
| Preparation method |
E. coli |
| Purity |
> 95% as determined by SDS-PAGE. |
| Alternative |
Inhibin beta A chain, Activin beta-A chain, Erythroid differentiation protein |
| Formulation |
Lyophilized from sterile PBS, pH 7.4 |
| Features & Benefits |
Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight |
13.1 kDa |
| Usage notes |
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions |
Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles. |
| Shipping |
The product is shipped with blue ice. |
| Precautions |
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
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