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Human M-CSF Protein, His tag (Animal-Free)

Human M-CSF Protein, His tag (Animal-Free)

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Specification

Product name Human M-CSF Protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from Human M-CSF (Glu33-Ser190) (P09603) was expressed with 6×His tag at the C-terminus
Applications His tag
Applications notes Human
Activity Measured by its ability to induce proliferation in NFS-60 cells.The ED50 for this effect is <1 ng/mL.The specific activity of recombinant human M-CSF is approximately > 2.5x 108IU/mg
Protein length The recombinant Human M-CSF consists of 158 amino acids and predicts a molecular mass of 19.76 kDa
Preparation method E. coli
Purity >98% as determined by SDS-PAGE.
Alternative CSF-1, MGI-IM

Product Properties

Formulation Lyophilized from PBS, pH 7.4
Features & Benefits Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
Molecular weight 19.76 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration not less than 100 ug/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously
Storage instructions Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Macrophage Colony Stimulating Factor (M-CSF) produced by osteoblasts and osteoblast precursors. M-CSF stimulates the growth and differentiation of the monocyte lineage, and promotes the survival, proliferation, and functions of mature monocytes/macrophages
Alternative CSF-1, MGI-IM
Accession P09603

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