| Product name | Human IL-8 protein |
| Sequence | Amino acid sequence derived from Human IL-8 isoform (P10145)(Ser28-Ser99) was expressed. |
| Activity | Immobilized human IL-8 at 200 ng/mL (100μL/well) can bind mouse anti-IL-8 antibody, the EC50 of mouse anti-IL-8 antibody is 66.4 ng/mL. |
| Protein length | The recombinant Human IL-8 consists of 72 amino acids (N-Met) and has a predicted molecular mass of 8 kDa. The apparent molecular mass of the Human IL-8 protein is approximately 8 kDa in SDS-PAGE under reducing conditions . |
| Preparation method | E. coli |
| Purity | ≥ 98 % as determined by SDS-PAGE |
| Formulation | Lyophilized from sterile 20 mM Tris, 200 mM NaCl pH 7.0. |
| Molecular weight | 8 kDa |
| Usage notes | Always centrifuge tubes before opening . It is recommended to centrifuge at 12,000 rpm for 1 minute. Then reconstitute the lyophilized Human IL-8 protein with the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions |
| Storage instructions | Lyophilized Human IL-8 proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.124% HSA or BSA). Please prevent freeze-thaw cycles. |
| Shipping | The product is shipped at ambient temperature. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Interleukin-8 (IL-8), also known as CXCL8, GCP-1, and NAP-1,belongs to the neutrophil-specific CXC family of chemokines.This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli,in response to an inflammatory stimulus and acts by recruiting neutrophils, T-cells and basophils to the site of inflammation. |
| Gene ID | 3576 |
| Accession | P10145 |
Fig.SDS-PAGE analysis of Human IL-8 protein
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