| Product name | Human IL-7 Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Human IL-7 (Asp26-His177) (P13232) was expressed with 6×His tag at the C-terminus |
| Applications | His tag |
| Applications notes | Human |
| Activity | Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBMC).The ED₅₀ for this effect is <0.8 ng/mL.The specific activity of recombinant human IL-7 is > 1 x 10⁸ IU/mg. |
| Protein length | The recombinant Human IL-7 Protein consists of 152 amino acids and predicts a molecular mass of 18.3 kDa. The protein migrates as 19 kDa under reducing condition (SDS-PAGE analysis). |
| Preparation method | E. coli |
| Purity | > 95% as determined by SDS-PAGE. |
| Alternative | Lymphopoietin 1(LP-1), pre-B-cell factor |
| Formulation | Lyophilized from sterile PBS, pH 8.0 |
| Features & Benefits | Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | 18.3 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL-7 Protein, His tag (Animal-Free) using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles. |
| Shipping | The product is shipped with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Interleukin-7 (IL-7) is a multipotent cytokine belonged to one of the members of IL-2 superfamily. IL-7 has diverse effects on the hematopoietic and immune regulations. IL-7 is a trophic factor that is necessary for both B cell and T cell proliferation and development. In addition, it presents potential antitumor effects in tumors such as glioma, melanoma, lymphoma, leukemia, prostate cancer, and glioblastoma. |
| Alternative | Lymphopoietin 1(LP-1), pre-B-cell factor |
| Accession | P13232 |
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