| Product name | Human IL-4 protein |
| Sequence | Amino acid sequence derived from Human IL-4 (His25-Ser153) (P05112-1) was expressed. |
| Applications | No tag |
| Applications notes | Human |
| Activity | Measured in a cell proliferation assay using TF -1. The ED50 for this effect is typically 0.144 ng/mL. The specific activity of recombinant Human IL4 is approximately >6.94 x 106IU/mg |
| Protein length | The recombinant Human IL-4 consists of 129 amino acids and predicts a molecular mass of 15.1 kDa. |
| Preparation method | E.coli |
| Purity | > 96 % as determined by SDS-PAGE |
| Molecular Formula | 15.1 kDa |
| Formulation | Lyophilized from sterile 20 mM Tris-HCl, 150 mM NaCl, pH 7.4. |
| Molecular weight | 15.1 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage instructions | Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Human Interleukin-4 (IL-4) is a cytokine that plays an important role in regulating inflammation and immune responses. It induces the differentiation of T cells to Th2 cells. This cytokine binds the IL-4 receptor that also binds another cytokine interleukin 13 (IL-13), which may explain the overlapping functions of IL-4 and IL-13. IL-4, originally designated as B-cell growth factor-1 (BSFl), is described in the supernatant of activated EL-4 thymoma cells as a factor that can co-stimulate B-cells activated with submitogenic concentrations of anti-IgM. |
| Gene ID | 3565 |
| Accession | P05112 |
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