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Human IL-37 Protein, His tag (Animal-Free)

Human IL-37 Protein, His tag (Animal-Free)

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Specification

Product name Human IL-37 Protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from Human IL-37 (Lys53-Asp218) (Q9NZH6) was expressed with 6×His tag at the C-terminus.
Applications His tag
Applications notes Human
Activity Measure by its ability to induce IL-8 secretion in human PBMCs.The ED₅₀ for this effect is <0.9 μg/mL.
Protein length The recombinant Human IL-37 Protein consists of 166 amino acids and predicts a molecular mass of 18.7 kDa
Preparation method E. coli
Purity > 95% as determined by SDS-PAGE.
Alternative IL-1F7, IL-1ζ (zeta), IL-1H4, FIL1, FIL1Z

Product Properties

Formulation The protein was lyophilized from sterile PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
Features & Benefits Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
Molecular weight 18.7 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously
Storage instructions Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles
Shipping The product is shipped with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Humaninterleukin-1 family member 7 (IL-37) is a member of interleukin-1 cytokine family. It is reported that five alternative splicing transcript variants encoding different subtypes have different expression profiles. The longest IL-37 transcript is called IL-37b or IL-37 isoform 1, which encodes a propeptide with 218 amino acid residues and a propeptide with 45 amino acids, and is cleaved to produce a mature protein. IL37b binds to IL18 Rα with low affinity, but does not produce any IL-18 excitation or antagonism
Alternative IL-1F7, IL-1ζ (zeta), IL-1H4, FIL1, FIL1Z
Accession Q9NZH6

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