| Product name |
Human IL-36RA Protein, His tag (Animal-Free) |
| Sequence |
Amino acid sequence derived from Human IL-36RA (Met1-Asp155) (Q9UBH0) was expressed with 6×His tag at the C-terminus. |
| Applications |
His tag |
| Applications notes |
Human |
| Activity |
Measure by its ability to inhibit IL-36 gamma-induced IL-8 secretion in PBMC cells. The ED₅₀ for this effect is <2 ng/mL in the presence of 500 ng/mL of recombinant human IL-36 gamma. |
| Protein length |
The recombinant Human IL-36RA consists of 155 amino acids and predicts a molecular mass of 17.77 kDa. |
| Preparation method |
E.coli |
| Purity |
> 98 % as determined by SDS-PAGE |
| Molecular Formula |
17.77 kDa |
| Alternative |
interleukin 36 receptor antagonist, FIL1 delta, IL-1F5, IL-1HY1, IL-1L1, IL-1RP3, IL-1ra Homolog 1, IL-1 delta, IL36RA |
| Formulation |
Lyophilized from sterile PBS，pH 7.4. |
| Features & Benefits |
<0.1 EU per μg of the protein as determined by the LAL method. |
| Molecular weight |
17.77 kDa |
| Usage notes |
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously. |
| Storage instructions |
Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA,10% FBS or 0.1%BSA). Please prevent freeze-thaw cycles. |
| Shipping |
Gel pack with blue ice. |
| Precautions |
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
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