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Human IL-25 Protein, His tag (Animal-Free)

Human IL-25 Protein, His tag (Animal-Free)

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Specification

Product name Human IL-25 Protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from Human IL-25 (Tyr33-Gly177) (Q8IU54) was expressed with 6×His tag at the C-terminus
Applications His tag
Applications notes Human
Activity Measured by its ability to induce IL-8 secretion in human PBMCs. The ED50 for this effect is <5 ng/mL. Measure by its ability to induce CXCL1 secretion in HT29 cells. The ED50 for this effect is <1 ng/mL
Protein length The recombinant Human IL-25 consists of 145 amino acids and predicts a molecular mass of 16.7 kDa
Preparation method E. coli
Purity >98% as determined by SDS-PAGE.
Alternative IL17E

Product Properties

Formulation Lyophilized from PBS, pH 8.0
Features & Benefits Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
Molecular weight 16.7 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Interleukin 25 (IL-25) is a cytokine with proinflammatory and anti-inflammatory effects, it predicts a molecular mass of 16.7 kDa. It can induce NF-κB activation, and stimulate the production of IL-8, which is the major chemotactic substance of neutrophils.
Alternative IL17E
Accession Q9H293

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