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Human IL-22 Protein, His tag (Animal-Free)

Human IL-22 Protein, His tag (Animal-Free)

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Specification

Product name Human IL-22 Protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from Human IL-22 (Ala 33-Ile 179) (Q9GZX6) was expressed with 6×His tag at the C-terminus
Applications His tag
Applications notes Human
Activity Measure by its ability to induce proliferation in A549 cells. The ED₅₀ for this effect is <0.5 ng/mL.
Protein length The recombinant Human IL-22 Protein consists of 147 amino acids and predicts a molecular mass of 16.9 kDa
Preparation method E. coli
Purity > 98% as determined by SDS-PAGE.
Alternative IL-TIF, Cytokine ZCYTO18

Product Properties

Formulation The protein was lyophilized from sterile PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
Features & Benefits Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
Molecular weight 16.9 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously
Storage instructions Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Interleukin 22(IL-22) is an α-helical cytokine, predicts a molecular mass of 16.9 kDa. It is produced by T-helper (Th)-17 cells, γδ T cells, NKT cells and newly described innate lymphoid cells (ILCs). Effects involve stimulation of cell survival, proliferation and synthesis of antimicrobials including S120, Reg3β, Reg3γ and defensins.
Alternative IL-TIF, Cytokine ZCYTO18
Accession Q9GZX6

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