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Human IL-17A protein, His tag (Animal-Free)

Human IL-17A protein, His tag (Animal-Free)

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Specification

Product name Human IL-17A protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from Human IL-17A protein (ILE20-Ala155) (Q16552) with 6×His tag at the C-terminus.
Applications His tag
Applications notes Human
Activity Measure by its ability to induce IL-6 secretion in 3T3 cells.The ED₅₀ for this effect is <6 ng/mL.
Protein length The secreted Human IL-17A protein consists of 136 amino acids and has a predicted molecular mass of 16.5 kDa
Preparation method E. coli
Purity >98% as determined by SDS-PAGE.
Alternative CTLA-8

Product Properties

Formulation Lyophilized from sterile PBS, pH 8.0
Features & Benefits Endotoxin:<0.01 EU per 1 μg of the protein by the LAL method.
Molecular weight The protein has a calculated MW of 16.47 kDa.The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Human IL-17A protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously
Storage instructions Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Interleukin 17A (IL-17A) predicts a molecular mass of 35 kDa, encoded IL17A gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappa B and mitogen-activated protein kinases.
Alternative CTLA-8
Accession Q16552

Image & description

Fig.SDS-PAGE analysis of Human IL-17A protein

Fig.SDS-PAGE analysis of Human IL-17A protein

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