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Human IL-12 p70 Protein, His tag (Animal-Free)

Human IL-12 p70 Protein, His tag (Animal-Free)

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Specification

Product name Human IL-12 p70 Protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from Human IL-12 p70 (Arg23-Ser219) (P29459), (Ile23-Ser328) (P29460) was expressed with 6×His tag at the C-terminus
Applications His tag
Applications notes Human
Activity Measured by its ability to induce IFN gamma secretion in PHA-activated human peripheral blood lymphocytes (PBMC).The ED50 for this effect is 0.05-0.2 ng/mL
Protein length The recombinant Human IL-12 p70 consists of 503 amino acids and predicts a molecular mass of 59.55 kDa
Preparation method E. coli
Purity >98% as determined by SDS-PAGE.
Alternative Interleukin-12, NKSF,Cytotoxic Lymphocyte Maturation Factor (CLMF), TSF

Product Properties

Formulation Lyophilized from PBS, pH 7.4
Features & Benefits Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
Molecular weight 59.55 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously
Storage instructions Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Interleukin 12 p70 (IL-12 p70) is an interleukin which is major secreted from immune cells like dendritic cells, macrophages and neutrophils. IL-12 p70 is a 58.5 kDa protein containing 527 amino acid residues which is composed by two subunits (IL-12 p35 and IL-12 p40). IL-12 p70 induces the cytotoxic activity of immune cells like NK cells and T cells. IL-12 p70 binds its receptor (IL-12Rβ1 and IL-12Rβ2) that activates the Jak/STAT signaling pathway. IL-12 p70 also plays an important role in cancer model which inhibits the formation of new blood vessels through the production of IFN γ.
Alternative Interleukin-12, NKSF,Cytotoxic Lymphocyte Maturation Factor (CLMF), TSF
Accession P29459(p35), P29460( p40)

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