| Product name |
Human IL-12 p70 Protein, His tag (Animal-Free) |
| Sequence |
Amino acid sequence derived from Human IL-12 p70 (Arg23-Ser219) (P29459), (Ile23-Ser328) (P29460) was expressed with 6×His tag at the C-terminus |
| Applications |
His tag |
| Applications notes |
Human |
| Activity |
Measured by its ability to induce IFN gamma secretion in PHA-activated human peripheral blood lymphocytes (PBMC).The ED50 for this effect is 0.05-0.2 ng/mL |
| Protein length |
The recombinant Human IL-12 p70 consists of 503 amino acids and predicts a molecular mass of 59.55 kDa |
| Preparation method |
E. coli |
| Purity |
>98% as determined by SDS-PAGE. |
| Alternative |
Interleukin-12, NKSF,Cytotoxic Lymphocyte Maturation Factor (CLMF), TSF |
| Formulation |
Lyophilized from PBS, pH 7.4 |
| Features & Benefits |
Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight |
59.55 kDa |
| Usage notes |
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions |
Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles |
| Shipping |
Gel pack with blue ice. |
| Precautions |
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
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