| Product name | Human IL-10 Protein |
| Sequence | Amino acid sequence derived from IL10 Human (Ser19-Asn178) (P22301) was expressed. |
| Activity | Testing in Progress |
| Protein length | The recombinant Human IL-10 consists of 161 amino acids and predicts a molecular mass of 18.78KDa |
| Preparation method | E. coli |
| Purity | > 97 % as determined by SDS-PAGE |
| Alternative | CSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor; IL10; IL-10; IL 10 |
| Formulation | Lyophilized from sterile 20mM Tris，0.15M NaCl, pH 7.4. |
| Molecular weight | 18.78 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Human IL-10 Protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions |
| Storage instructions | Lyophilized recombinant Human IL-10 Protein product should be stored desiccated below-20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below-20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Shipping | The product is shipped at ambient temperature. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | IL-10 is an anti-inflammatory cytokine that belongs to the IL-10 family. It is produced by a variety of cell lines, including T-cells, macrophages, mast cells, and other cell types, while it is produced primarily by monocytes and to a lesser extent by lymphocytes. IL-10 is mainly expressed in monocytes and Type 2 T helper cells (TH2), mast cells,CD4+CD25+Foxp3+ regulatory T cells, and also in a certain subset of activated T cells and B cells. IL-10 has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. The importance of interleukin 10 for counteracting excessive immunity in the human body is revealed by the fact that patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant IL-10. IL-10 inhibits the synthesis of some cytokines, including IFN-gamma, IL-2, IL-3, TNF, and GM-CSF produced by activated macrophages and by helper T-cells. It also displays a potent ability to suppress the antigen-presentation capacity of antigen-presenting cells. However, it is also stimulatory towards certain T cells and mast cells and stimulates B cell maturation and antibody production |
| Gene ID | 3586 |
| Alternative | CSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor; IL10; IL-10; IL 10 |
| Accession | P22301 |
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