| Product name | Human IL-10 Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Human IL-10 (Ser19-Asn178)(P22301) was expressed with 6×His tag at the C-terminus |
| Applications | His tag |
| Applications notes | Human |
| Activity | Measure by its ability to induce MC/9‑2 cells proliferation.The ED₅₀ for this effect is <1 ng/mL.The specific activity of recombinant human IL-10 is approximately >1x10⁶ IU/ mg. |
| Protein length | The recombinant Human IL-10 consists of 161 amino acids and predicts a molecular mass of 18.8 kDa. |
| Preparation method | E. coli |
| Purity | >98% as determined by SDS-PAGE. |
| Alternative | B-TCGF, CSIF, TGIF |
| Formulation | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us. |
| Features & Benefits | Endotoxin:<0.01 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | The protein has a calculated MW of 19.6 kDa. The protein migrates as 20 kDa under reducing condition (SDS-PAGE analysis). |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant human IL-10 protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Interleukin-10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. Many different types of cells can produce IL-10, including immune cells and non-immune cells. IL-10 exerts inhibitory functions on DCs and macrophages, which is a potent inhibitor of antigen presentation and limit the production of the Th1-associated cytokines IL-2 and interferon-γ (IFN-γ). IL-10 is also a key immunoregulator during infection due to its inhibitory effect on inflammatory cytokine production. Consequently, the excessive Th1 and CD8+ T cell responses could be suppressed during infection. |
| Alternative | B-TCGF, CSIF, TGIF |
| Accession | P22301 |
Fig.SDS-PAGE analysis of Human IL-10 protein
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