| Product name |
Human IL-1β protein, His tag (Animal-Free) |
| Sequence |
Amino acid sequence derived from Human IL-1β (Gly21-Asn132)(NP_000567.1) was expressed with 6×His tag at the C-terminus |
| Applications |
His tag |
| Applications notes |
Human |
| Activity |
Measure by its ability to induce IL-8 secretion in HT29 cells. The ED₅₀ for this effect is <2.0 ng/mL. The specific activity of recombinant human IL-1 beta is approximately >3 x 10⁷ IU/mg. |
| Protein length |
The secreted Human IL-1β consists of 112 amino acids and has a predicted molecular mass of 18.5 kDa |
| Preparation method |
E. coli |
| Purity |
>98% as determined by SDS-PAGE. |
| Alternative |
Catabolin, Lymphocyte-Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF) |
| Formulation |
Lyophilized from sterile PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us. |
| Features & Benefits |
Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight |
The protein has a calculated MW of 18.5 kDa.
The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis). |
| Usage notes |
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL-1β protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions |
Lyophilized Human IL-1β protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it
is recommended to add a carrier protein (0.1% BSA, 10% FBS or 5% HSA). Please prevent freeze-thaw cycles |
| Shipping |
Gel pack with blue ice. |
| Precautions |
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
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