| Product name | Human IFN-β1a protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Human IFN-β1a protein (Met30-Asn187)(P01574) with 6×His tag at the C-terminus was expressed |
| Applications | His tag |
| Applications notes | Human |
| Activity | Measure by its ability to induce apoptosis in HeLa cells.The ED₅₀ for this effect is <15 ng/mL.Measure by its ability to induce cytotoxicity in TF-1 cells.The ED₅₀ for this effect is <0.1 ng/mL.The specific activity of recombinant human IFN beta 1a is approximately >1 x10⁷ IU/ mg. |
| Protein length | The secreted Human IFN-β1a protein consists of 158 amino acids and has a predicted molecular mass of 20.8 kDa |
| Preparation method | E. coli |
| Purity | >98% as determined by SDS-PAGE. |
| Alternative | IFNB1, Type I Interferon |
| Formulation | Lyophilized from sterile PBS, pH 8.0 |
| Features & Benefits | Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | The protein has a calculated MW of 20.84 kDa.The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis). |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions | Lyophilized protein protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Interferon-beta (IFN-β1a), acting via STAT1 and STAT2, is known to upregulate and downregulate a wide variety of genes, most of which are involved in the antiviral immune response. It is a member of TypeⅠ IFNs, which include IFN-α, -β, τ, and-ω. IFN-β plays an important role in inducing non-specific resistance against a broad range of viral infections. It also affects cell proliferation and modulates immune responses |
| Alternative | IFNB1, Type I Interferon |
| Accession | P01574 |
Fig.SDS-PAGE analysis of Human IFN-β1a protein
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