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Human IFN-α1a Protein, His tag (Animal-Free)

Human IFN-α1a Protein, His tag (Animal-Free)

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Specification

Product name Human IFN-α1a Protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from Human IFN α1a (Cys 24-Glu 189) (P01562) was expressed with 6×His tag at the C-terminus
Applications His tag
Applications notes Human
Activity Measure by its ability to inhibit IL-8 secretion in human PBMCs in the presence of LPS.The ED₅₀ for this effect is <1.12 μg /mL.
Protein length The recombinant Human IFN-α1a Protein consists of 166 amino acids and predicts a molecular mass of 19.4 kDa
Preparation method E. coli
Purity > 95% as determined by SDS-PAGE.
Alternative Leukocyte Interferon, IFNA2, B cell Interferon, Type I Interferon

Product Properties

Formulation The protein was lyophilized from sterile PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
Features & Benefits Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
Molecular weight 19.4 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously
Storage instructions Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles
Shipping The product is shipped with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Interferon-alpha 1a (IFN-alpha 1a) is a leukocyte interferon, which is a variant of Interferon-alpha. IFN-alpha 1a is a 19.5kDa protein containing 167 amino acid residues. It could bind the interferon receptors that activates the signal transduction of immune responses through the Jak/STAT pathway in leukocytes and lymphoblastoid cells.
Alternative Leukocyte Interferon, IFNA2, B cell Interferon, Type I Interferon
Accession P01562

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