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Human GDNF protein, His tag (Animal-Free)

Human GDNF protein, His tag (Animal-Free)

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Specification

Product name Human GDNF protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from Human GDNF protein (Ser78-Ile211) with 6×His tag at the C-terminus was expressed.
Applications His tag
Applications notes Human
Activity Measure by its ability to induce proliferation in SH-SY5Y cells.The ED₅₀ for this effect is <10 ng/mL.
Protein length The recombinant Human GDNF consists of 134 amino acids and predicts a molecular mass of 16 kDa
Preparation method E. coli
Purity >95% as determined by SDS-PAGE.
Alternative ATF, ATF1, ATF2, HFB1-GDNF, HSCR3

Product Properties

Formulation The protein was lyophilized from 20 mM sodium citrate, 0.2 M NaCl, pH 3.5. If you have any concerns or special requirements, please confirm with us.
Features & Benefits Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method.
Molecular weight The protein has a calculated MW of 16.01 kDa. The protein migrates as 16 kDa under reducing condition (SDS-PAGE analysis).
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Human GDNF protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously
Storage instructions Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Glial cell line-derived neurotrophic factor (GDNF) is the neurotrophic factor, belonging to the GDNF family of ligands (GFL) and identifying as a therapeutic candidate in Parkinson's disease. GDNF is a 23.7 kDa protein containing 211 residues that plays a critical role in promoting the survival and differentiation of midbrain dopamine neurons. Besides, GDNF is revealed to facilitate the development of peripheral tissues such as kidney, pancreas and lung. Additionally, as a member of GFL, GDNF also takes part in the progression of tumor.
Alternative ATF, ATF1, ATF2, HFB1-GDNF, HSCR3
Accession P39905

Image & description

Fig.SDS-PAGE analysis of Human GDNF protein

Fig.SDS-PAGE analysis of Human GDNF protein

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