| Product name | Human GDF3 Protein |
| Sequence | Amino acid sequence derived from Human GDF 3(Q9NR23) (Ala251-Gly364) was expressed. |
| Applications | No tag |
| Applications notes | Human |
| Activity | Testing in progress |
| Protein length | The recombinant Human GDF 3 consists of 141 amino acids and predicts a molecular mass of 13 kDa |
| Preparation method | E. coli |
| Purity | > 96% as determined by SDS-PAGE |
| Alternative | C78318; ecat9; GDF 3; GDF-3; GDF3; GDF3_HUMAN; Growth differentiation factor 3; Growth/differentiation factor 3; KFS3; MCOP7; MCOPCB6; MGC123990; MGC123991; RGD1564178; Vgr 2; Vgr2; |
| Formulation | Lyophilized from 20mM Tris 150mM NaCl PH 8.0 |
| Features & Benefits | Endotoxin: < 1 EU per µg of the protein as determined by the LAL method. |
| Molecular weight | 13 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to diluted the protein using the PBS buffer or Protein storage solution to working concentration |
| Storage instructions | The recombinant Human GDF 3 protein product should be stored desiccated below -20°C.The protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | GDF-3 (previously called Vgr-2) is a TGF-beta superfamily member belonging to the growth/differentiation factor family. GDF-3 is expressed in undifferentiated embryonic stem (ES) cells, white adipose tissue and the brain. The 366 amino acid (aa) mouse GDF-3 contains a 22 aa signal sequence, a 230 aa propeptide and a 114 aa mature protein that contains one potential N-glycosylation site. The mature region contains a cysteine-knot structure that is conserved throughout family members. However, it lacks the fourth cysteine which is responsible for the formation of an inter-molecular disulfide bond, so GDF-3 may exist as a non-covalent homodimer. Mature human GDF-3 shares 83%, 83% aa sequence identity with mouse and rat GDF-3. Most of GDF-3 is present as the uncleaved prepro form. The uncleaved and the mature forms both appear to have activity, but that activity may differ. All forms can oppose BMPs. In ES cells, inhibition of BMP2 signaling by GDF-3 maintains pluripotency. GDF-3 also influences early cell fate decisions; for example, deletion of mouse GDF-3 produces defects in the anterior visceral endoderm of the pre-gastrulation embryo. GDF-3 cooperates with GDF-1 in embryogenesis, and the mature protein has nodal-like activity. Although GDF family members signal through BMP receptors (ALK1, 2, 3 and 6), which activate Smads 1, 5 and 8, GDF-3 signaling through ALK4 and ALK7, which activate Smads 2 and 3, has also been reported. In adipocytes, GDF-3 is induced by a high fat diet, promoting adipogenesis and obesity |
| Gene ID | 9573 |
| Alternative | C78318; ecat9; GDF 3; GDF-3; GDF3; GDF3_HUMAN; Growth differentiation factor 3; Growth/differentiation factor 3; KFS3; MCOP7; MCOPCB6; MGC123990; MGC123991; RGD1564178; Vgr 2; Vgr2; |
| Accession | Q9NR23 |
Fig.SDS-PAGE analysis of Human GDF3 Protein
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