| Product name | Human G-CSF Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Human G-CSF (Thr31-Pro204) (NP_757373) was expressed with 6×His tag at the N-terminus |
| Applications | His tag |
| Applications notes | Human |
| Activity | Measure by its ability to induce proliferation in NFS-60 cells.The ED₅₀ for this effect is <50 pg/mL. The specific activity of recombinant human G-CSF is > 2 x 10⁷ IU/mg. |
| Protein length | The recombinant Human G-CSF Protein consists of 174 amino acids and predicts a molecular mass of 19.48 kDa |
| Preparation method | E. coli |
| Purity | > 97% as determined by SDS-PAGE. |
| Alternative | CSF-3, MGI-1G, GM-CSF beta, pluripoietin |
| Formulation | Lyophilized from sterile PBS, pH7.4 |
| Features & Benefits | Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | 19.48 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles |
| Shipping | The product is shipped with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of glycoproteins and makes the bone marrow produce more white blood cells so it can reduce the risk of infection after some types of cancer treatment also is an established useful clinical agent for increasing neutrophilic granulocytes levels. G-CSF is a 18.67 kDa protein having 207 amino acid which secreted by monocytes, macrophages, fibroblasts, and endothelial cells, regulates cell growth, maturation, development of myeloid cells. |
| Alternative | CSF-3, MGI-1G, GM-CSF beta, pluripoietin |
| Accession | NP_757373 |
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