| Product name | Human Flt-3 Ligand protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Human Flt-3 Ligand (Thr27-Ala181)(P49771) was expressedwith 6×His tag at the C-terminus |
| Applications | His tag |
| Applications notes | Human |
| Activity | Measure by its ability to induce proliferation in BaF3 cells transfected with mouse Flt-3.The ED₅₀ for this effect is <0.8 ng/mL.The specific activity of recombinant human Flt-3 Ligand is > 1.5 x 10⁶ IU/mg. |
| Protein length | The secreted Human Flt-3 Ligand consists of 155 amino acids and has a predicted molecular mass of 17.6 kDa |
| Preparation method | E. coli |
| Purity | >95% as determined by SDS-PAGE. |
| Alternative | FL, FLG3L, FLT3L |
| Formulation | Lyophilized from sterile PBS, pH 8.0 |
| Features & Benefits | Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | The protein has a calculated MW of 18.6 kDa. The protein migrates as 19 kDa under reducing condition (SDS-PAGE analysis). |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Human Flt-3 Ligand protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Fms-like Tyrosine Kinase 3 Ligand (Flt-3 Ligand), also known as FLT3L, is a small molecule that acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. In vivo, Flt-3 Ligand also induces the mobilization of the hematopoietic progenitors and stem cells. This may help the system to kill cancer cells. Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. Flt-3 Ligand controls the development of DCs and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts |
| Alternative | FL, FLG3L, FLT3L |
| Accession | P49771 |
Fig.SDS-PAGE analysis of Human Flt-3 Ligand protein
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