| Product name | Human FGF-8a Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Human FGF-8a (Gln52-Arg233) (P55075) was expressed with 6×His tag at the C-terminus. |
| Applications | His tag |
| Applications notes | Human |
| Activity | Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <2 μg/mL. |
| Protein length | The recombinant Human FGF-8a consists of 182 amino acids and predicts a molecular mass of 22.14 kDa. |
| Preparation method | E.coli |
| Purity | > 98 % as determined by SDS-PAGE |
| Molecular Formula | 22.14 kDa |
| Alternative | fibroblast growth factor 8a, AIGF, HBGF-8, FGF8 |
| Formulation | Lyophilized from sterile PBS，pH 8.0. |
| Features & Benefits | Endotoxin :< 0.1 EU per 1 μg of the protein as determined by the LAL method. |
| Molecular weight | 22.14 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously. |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA,10% FBS or 0.1%BSA). Please prevent freeze-thaw cycles. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | FGF-8 is a heparin binding growth factor belonging to the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. There are 4 known alternate spliced forms of FGF8; FGF-8A, FGF-8B, FGF-8E and FGF-8F. The human and murine FGF-8A and B are identical, unlike human and mouse FGF-8E and F, which are 98% identical. FGF-8 targets mammary carcinoma cells and other cells expressing the FGF receptors. |
| Gene ID | 2253 |
| Alternative | fibroblast growth factor 8a, AIGF, HBGF-8, FGF8 |
| Accession | P55075 |
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