Login Register
English
0

Cart

$ 0

Human FGF-21 Protein, His tag (Animal-Free)

Human FGF-21 Protein, His tag (Animal-Free)

Views(725) Publications(0) Catalog no(PRP1094)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Human FGF-21 Protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from Human FGF-21 (His29-Ser209) (Q9NSA1) was expressed with 6×His tag at the C-terminus
Applications His tag
Applications notes Human
Activity Measure by its ability to induce proliferation in BaF3 cells transfected with human FGFRIIIc.The ED₅₀ for this effect is <0.4 μg/mL.
Protein length The protein has a calculated MW of 21.35 kDa. The protein migrates as 25 kDa under reducing condition (SDS-PAGE analysis).
Preparation method E. coli
Purity > 98% as determined by SDS-PAGE.
Alternative FGFL

Product Properties

Formulation Lyophilized from sterile PBS, pH8.0
Features & Benefits Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
Molecular weight 21.35 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.
Storage instructions Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles
Shipping The product is shipped with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Fibroblast Growth Factors-21 (FGF-21) is a 22.3 kDa member of the fibroblast Growth Factors with 209 amino acid residues. FGF-21 is expressed from liver and cardiomyocytes. FGF-21 is a key protein that regulates important metabolic pathways, and modulates cellular function, metabolism, and senescence. It can stimulate glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1 /GLUT1 expression.
Alternative FGFL
Accession Q9NSA1

Image & description

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Human FGF-21 Protein, His tag (Animal-Free)”