| Product name | Human FGF-2（154 aa）protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Human FGF-2 protein (Ala135-Ser288) was expressed |
| Applications | His tag |
| Applications notes | Human |
| Activity | Measure by its ability to induce 3T3 cells proliferation.The ED₅₀ for this effect is <1 ng/mL.The specific activity of recombinant human FGF-2 is approximately >5 x 10⁵ IU/mg. |
| Protein length | The Human FGF-2 protein consists of 154 amino acids and has a predicted molecular mass of 17 kDa |
| Preparation method | E. coli |
| Purity | >98% as determined by SDS-PAGE. |
| Alternative | HBGF-2, Prostatropin |
| Formulation | Lyophilized from sterile 0.01% sarkosyl in PBS, pH 8.0.If you have any concerns or special requirements, please confirm with us. |
| Features & Benefits | Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | The protein has a calculated MW of 18.1 kDa. The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis). |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions | Lyophilized Human FGF-2 protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Basic fibroblast Growth Factors (FGF-2, bFGF, FGF-β), a 18 kDa pleiotropic cytokine, plays multiple roles in different cells and tissues. FGF-2 can stimulate smooth muscle cell growth, wound healing, and tissue repair. In addition, FGF-2 has been shown to regulate the generation of neurons and astrocytes from progenitor cells. FGF-2 are also involved in a variety of biological processes, including embryonic development, morphogenesis, tissue repair, tumor growth, and invasion. As a multifunctional cytokine, FGF-2 is first isolated from the pituitary. Later, it was identified from various cell types including cardiac myocytes, cardiac fibroblasts, endothelial cells, and smooth muscle cells. |
| Alternative | HBGF-2, Prostatropin |
| Accession | P09038 |
Fig.SDS-PAGE analysis of Human FGF-2（154 aa）protein
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