| Product name | Human EGF protein |
| Sequence | MNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR with polyhistidine tag at the C-terminus |
| Activity | Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is < 1.0 ng/mL. The specific activity of recombinant human EGF is approximately >1.0 x 10⁶ IU/mg. |
| Protein length | The protein has a calculated MW of 7.16 kDa.The protein migrates as 9-11 kDa under reducing condition (SDS-PAGE analysis). |
| Preparation method | E. coli |
| Purity | >95% as determined by SDS-PAGE. |
| Alternative | Urogastrone, URG |
| Formulation | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us. |
| Features & Benefits | Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | The protein has a calculated MW of 7.16 kDa.The protein migrates as 9-11 kDa under reducing condition (SDS-PAGE analysis). |
| Usage notes | Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
| Storage instructions | Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Human epidermal Growth Factors (EGF) is a 6 kDa cytokine with 53 amino acid residues. EGF is mainly secreted from ectodermal cells, monocytes, kidney and duodenal glands. Upon binding to its receptor, EGFR, EGF acts to stimulate cell growth and proliferation of epithelial cells, play important roles in many developmental processes including accelerate tooth eruption, inhibits gastric acid secretion, and involve in wound healing. |
| Alternative | Urogastrone, URG |
| Accession | P01133 |
Fig.SDS-PAGE analysis of Human EGF protein
Author：Fang, Tian, et al. Publication name：Breast Cancer Research and Treatment IF：3
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