| Product name | Human CXCL13 Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Human CXCL13 (Val23-Arg94) (O43927) was expressed with 6×His tag at the N-terminus. |
| Applications | His tag |
| Applications notes | Human |
| Activity | Measure by its ability to chemoattract BaF3 cells transfected with human CXCR13.The ED₅₀ for this effect is <20 ng/mL. |
| Protein length | The recombinant Human CXCL13 consists of 72 amino acids and predicts a molecular mass of 9.49 kDa. |
| Preparation method | E.coli |
| Purity | > 98 % as determined by SDS-PAGE |
| Molecular Formula | 9.49 kDa |
| Alternative | B-cell Attracting Chemokine-1, BLR1 Ligand, BCA-1/BLC |
| Formulation | Lyophilized from sterile PBS，pH 7.4. |
| Features & Benefits | Endotoxin :< 0.1 EU per μg of the protein as determined by the LAL method. |
| Molecular weight | 9.49 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously. |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA,10% FBS or 0.1%BSA). Please prevent freeze-thaw cycles. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | CXCL13, also known as BCA-1 (B Cell-Attracting chemokine 1) or BLC, , is a recently identified new CXC chemokines. This chemokine is expressed in the stomach, spleen, liver, appendix and lymph nodes. CXCL13 can only elicit its activities through CXCR5 receptor. BCA-1/BLC is a potent chemoattractant for B lymphocytes, and induces weak chemotactic response in T cells and macrophages. It should be noticed that CXCL13 shows no activity on neutrophils and monocytes |
| Gene ID | 10563 |
| Alternative | B-cell Attracting Chemokine-1, BLR1 Ligand, BCA-1/BLC |
| Accession | O43927 |
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