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Human CXCL13 Protein, His tag (Animal-Free)

Human CXCL13 Protein, His tag (Animal-Free)

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Specification

Product name Human CXCL13 Protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from Human CXCL13 (Val23-Arg94) (O43927) was expressed with 6×His tag at the N-terminus.
Applications His tag
Applications notes Human
Activity Measure by its ability to chemoattract BaF3 cells transfected with human CXCR13.The ED₅₀ for this effect is <20 ng/mL.
Protein length The recombinant Human CXCL13 consists of 72 amino acids and predicts a molecular mass of 9.49 kDa.
Preparation method E.coli
Purity > 98 % as determined by SDS-PAGE
Molecular Formula 9.49 kDa
Alternative B-cell Attracting Chemokine-1, BLR1 Ligand, BCA-1/BLC

Product Properties

Formulation Lyophilized from sterile PBS,pH 7.4.
Features & Benefits Endotoxin :< 0.1 EU per μg of the protein as determined by the LAL method.
Molecular weight 9.49 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.
Storage instructions Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA,10% FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background CXCL13, also known as BCA-1 (B Cell-Attracting chemokine 1) or BLC, , is a recently identified new CXC chemokines. This chemokine is expressed in the stomach, spleen, liver, appendix and lymph nodes. CXCL13 can only elicit its activities through CXCR5 receptor. BCA-1/BLC is a potent chemoattractant for B lymphocytes, and induces weak chemotactic response in T cells and macrophages. It should be noticed that CXCL13 shows no activity on neutrophils and monocytes
Gene ID 10563
Alternative B-cell Attracting Chemokine-1, BLR1 Ligand, BCA-1/BLC
Accession O43927

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