| Product name | Human CCL3 Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Human CCL3 (Ala27-Ala92) (P10147) was expressed with 6×His tag the N-terminus |
| Applications | His tag |
| Applications notes | Human |
| Activity | Measured by its ability to chemoattract human PBMC using a concentration range of 5-50 ng/mL |
| Protein length | The recombinant Human CCL3 consists of 66 amino acids and predicts a molecular mass of 8.4 kDa |
| Preparation method | E. coli |
| Purity | >95% as determined by SDS-PAGE. |
| Alternative | MIP-1a: Macrophage Inflammatory Protein-1α, LD78α |
| Formulation | Lyophilized from 50 mM Tris ,150 mM NaCl, pH 8.5 |
| Features & Benefits | Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | 8.4 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | C-C Motif Chemokine Ligand 3 (CCL3) is also called macrophage inflammatory protein 1-alpha (MIP-1-alpha) and is expressed in bone marrow and liver. Upon binding to the receptor, CCR1, CCR4, or CCR5, CCL3 plays an essential role in acute inflammatory responses like recruitment and activation of polymorphonuclear leukocytes and induction of monophasic fever. In addition, CCL3 participates in resistance to type 1 virus infection, mediation of MAPK signaling pathway, cell-cell signaling, and calcium-mediated signaling |
| Alternative | MIP-1a: Macrophage Inflammatory Protein-1α, LD78α |
| Accession | P10147 |
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