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Human BMP-4 Protein, His tag (Animal-Free)

Human BMP-4 Protein, His tag (Animal-Free)

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Specification

Product name Human BMP-4 Protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from Human BMP-4 (Met302-Arg408)(P12644) was expressed with 6×His tag at the C-terminus.
Applications His tag
Applications notes Human
Activity Measure by its ability to induce alkaline phosphatase production by ATDC5 cells.The ED₅₀ for this effect is <0.58 ng/mL.
Protein length The recombinant Human BMP-4 consists of 107 amino acids and predicts a molecular mass of 12.1 kDa
Preparation method E. coli
Purity >95% as determined by SDS-PAGE.
Alternative BMP-2B, DVR4

Product Properties

Formulation The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium carbonate, pH 9.0. If you have any concerns or special requirements, please confirm with us.
Features & Benefits Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method.
Molecular weight The protein has a calculated MW of 12.88 kDa. The protein migrates as 12 kDa under reducing condition (SDS-PAGE analysis).
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant human BMP-4 protein to a concentration of 0.1-1 mg/mL in sterile ddH O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously
Storage instructions Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Bone morphogenic protein-4 (BMP-4) is one of nine structurally related BMPs belonging to the transforming growth factor-beta (TGF-beta) superfamily of secreted proteins. Mature BMP-4 is a dimer that binds to a multimeric transmembrane receptor with serine/threonine kinase activity. BMP-4 has important roles as a signalling molecule in embryonic tissues, including the developing central and peripheral nervous system, musculature and skeleton. BMP-4 as well as its receptors and their signal transduction pathways play a crucial role in the control of the differentiation and proliferation of the different cell types that constitute the anterior pituitary
Alternative BMP-2B, DVR4
Accession P12644

Image & description

Fig.SDS-PAGE analysis of Human BMP-4 protein

Fig.SDS-PAGE analysis of Human BMP-4 protein

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