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Human BMP-2 Protein, His tag (Animal-Free)

Human BMP-2 Protein, His tag (Animal-Free)

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Specification

Product name Human BMP-2 Protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from Human BMP-2 (Gln283-Arg396)(P12643) was expressed with 6×His tag at the C-terminus
Applications His tag
Applications notes Human
Activity Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <1.0 μg/mL.
Protein length The recombinant Human BMP-2consists of 115 amino acids and predicts a molecular mass of 13 kDa
Preparation method E. coli
Purity >95% as determined by SDS-PAGE.
Alternative Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF)

Product Properties

Formulation The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5. If you have any concerns or special requirements, please confirm with us.
Features & Benefits Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method.
Molecular weight The protein has a calculated MW of 13.76 kDa. The protein migrates as 14 kDa under reducing condition (SDS-PAGE analysis).
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Human BMP-2 Protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously
Storage instructions Lyophilized protein protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Bone Morphogenetic Protein-2 (BMP-2) is an extracellular multifunctional signaling cytokine that is also a member of the TGF-β family. BMP-2 can bind with the TGF-β receptor to active SMAD protein signal transduction. Moreover, it engages in the path of signals such as Wnt and Notch. BMP-2 plays an essential role in the growth of hard bones and cartilage and involved in the differentiation of osteoblasts.
Alternative Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF)
Accession P12643

Image & description

Fig.SDS-PAGE analysis of Human BMP-2 protein

Fig.SDS-PAGE analysis of Human BMP-2 protein

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