| Product name | Human 4-1BBL Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Human 4-1BBL Protein (Arg71-Glu254) (P41273) was expressed with 6×His tag at the C-terminus. |
| Applications | His tag |
| Applications notes | Human |
| Activity | Measure by its ability to induce IL-8 secretion in human PBMCs. The ED₅₀ for this effect is 1-5 ng/mL. |
| Protein length | The recombinant Human 4-1BBL Protein consists of 184 amino acids and predicts a molecular mass of 20.4 kDa. |
| Preparation method | E.coli |
| Purity | > 95 % as determined by SDS-PAGE |
| Molecular Formula | 20.4 kDa |
| Alternative | 4-1BB ligand, CD137L, TNLG5A, TNFSF9, Tumor necrosis factor ligand superfamily member 9 |
| Formulation | Lyophilized from sterile PBS，pH 7.4. |
| Features & Benefits | <0.05 EU per μg of the protein as determined by the LAL method. |
| Molecular weight | 20.4 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously. |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA,10% FBS or 0.1%BSA). Please prevent freeze-thaw cycles. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | 4-1BB ligand (4-1BBL) is a type II transmembrane protein that is part of the tumor necrosis factor (TNF) ligand family. As an inducible co-stimulatory molecule, it presents on several antigen presenting cell (APC) types, including B cells, macrophages and DCs. The interactions between 4-1BB and 4-1BBL trigger pleiotropic effects on the immune response including antigen presenting process, proliferation of CD4 and CD8 positive T-cells, as well as cytokine secretion from T-cells through NFκB, c-Jun, and p38 downstream signal pathways activation.Therefore, 4-1BB and 4-1BBL are recently used for the immunotherapy of cancer. |
| Gene ID | 8744 |
| Alternative | 4-1BB ligand, CD137L, TNLG5A, TNFSF9, Tumor necrosis factor ligand superfamily member 9 |
| Accession | P41273 |
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