| Product name | Human β-NGF protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Human β-NGF (Ser122-Ala241)(P01138) was expressed with 6×His tag at the C-terminus |
| Applications | His tag |
| Applications notes | Human |
| Activity | Measure by its ability to induce TF-1 cells proliferation.The ED₅₀ for this effect is <0.7 ng/mL.The specific activity of recombinant human beta-NGF is > 1 x 10⁶ IU/mg. |
| Protein length | The secreted Human β-NGFconsists of 120 amino acids and has a predicted molecular mass of 13.5 kDa |
| Preparation method | E. coli |
| Purity | >98% as determined by SDS-PAGE. |
| Alternative | β-Nerve Growth Factor, NGF-β |
| Formulation | The secreted Human β-NGFconsists of 120 amino acids and has a predicted molecular mass of 13.5 kDa |
| Features & Benefits | Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | The protein has a calculated MW of 14.43 kDa. The protein migrates as 11 kDa under reducing condition (SDS-PAGE analysis). |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Human β-NGF protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Beta-Nerve Growth Factor (β-NGF), also known as Beta-NGF, NGF, NGFB, is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. β-NGF can also downregulate the production of IFN-γ by T cells. Recombinant β-NGF has been used in many clinical trials to treat different disease including Alzheimer's disease, diabetic polyneuropathy, and HIV-associated peripheral neuropathy |
| Alternative | β-Nerve Growth Factor, NGF-β |
| Accession | P01138 |
Fig.SDS-PAGE analysis of Human β-NGF protein
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