| Product name | HSF1 (phospho Ser303) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human HSF1 around the phosphorylation site of S303 |
| Host | Rabbit |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | HSF1; HSTF1; Heat shock factor protein 1; HSF 1; Heat shock transcription factor 1; HSTF 1 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 82kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The product of HSF1 is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of HSF1. |
| Gene ID | 3297 |
| Alternative | HSF1; HSTF1; Heat shock factor protein 1; HSF 1; Heat shock transcription factor 1; HSTF 1 |
| Others | Phospho-HSF1 (S303) Polyclonal Antibody detects endogenous levels of HSF1 protein only when phosphorylated at S303. |
| Accession | Q00613 |
| Observed Band(KD) | 82 |
Fig. Western Blot analysis of MCF7+TNF cells using Phospho-HSF1 (S303) Polyclonal Antibody diluted at 1:1000.
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