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hnRNP K (phospho Ser284) Polyclonal Antibody

hnRNP K (phospho Ser284) Polyclonal Antibody

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Specification

Product name hnRNP K (phospho Ser284) Polyclonal Antibody
Immunogen Synthesized peptide derived from human hnRNP K around the phosphorylation site of S284
Host Rabbit
Reactivity Human,Mouse,Rat,Monkey
Applications WB,IHC,IF,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:20000;IF 1:50-200
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative HNRNPK; HNRPK; Heterogeneous nuclear ribonucleoprotein K; hnRNP K; Transformation up-regulated nuclear protein; TUNP

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 51kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background HNRNPK belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized.
Gene ID 3190
Alternative HNRNPK; HNRPK; Heterogeneous nuclear ribonucleoprotein K; hnRNP K; Transformation up-regulated nuclear protein; TUNP
Others Phospho-hnRNP K (S284) Polyclonal Antibody detects endogenous levels of hnRNP K protein only when phosphorylated at S284.
Accession P61978
Observed Band(KD) 51

Image & description

Fig. Western Blot analysis of 3T3 cells using Phospho-hnRNP K (S284) Polyclonal Antibody.

Fig. Western Blot analysis of 3T3 cells using Phospho-hnRNP K (S284) Polyclonal Antibody.

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