| Product name | hnRNP F Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the N-terminal region of human hnRNP F at AA range: 30-110 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | HNRNPF; HNRPF; Heterogeneous nuclear ribonucleoprotein F; hnRNP F; Nucleolin-like protein mcs94-1 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 48kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | HNRNPF belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. Heterogeneous nuclear ribonucleoprotein F encoded by HNRNPF has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified. |
| Gene ID | 3185 |
| Alternative | HNRNPF; HNRPF; Heterogeneous nuclear ribonucleoprotein F; hnRNP F; Nucleolin-like protein mcs94-1 |
| Others | hnRNP F Polyclonal Antibody detects endogenous levels of hnRNP F protein. |
| Accession | P52597 |
| Observed Band(KD) | 48 |
Fig.1. Western Blot analysis of various cells using hnRNP F Polyclonal Antibody.
Fig.2. Western Blot analysis of HepG2 cells using hnRNP F Polyclonal Antibody.
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