| Product name | Histone H3.3 (phospho Ser31) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human Histone H3.3 around the phosphorylation site of S31 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. H3F3A contains introns and its mRNA is polyadenylated, unlike most histone genes. The H3 histone family member 3A encoded is a replication-independent member of the histone H3 family. |
| Gene ID | 3020/3021 |
| Alternative | H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3 |
| Others | Phospho-Histone H3.3 (S31) Polyclonal Antibody detects endogenous levels of Histone H3.3 protein only when phosphorylated at S31. |
| Accession | P84243 |
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