| Product name | Histone H2B Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human Histone H2B around the non-acetylation site of K12 at AA range: 10-90 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Monkey |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:5000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | H2BFS; Histone H2B type F-S; Histone H2B.s; H2B/s |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 14kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | H2BFS is a Pseudogene. Histone H2B type 1-H is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid. Diseases associated with H2BFS include endometrial stromal sarcoma. Among its related pathways are Packaging Of Telomere Ends. Gene Ontology (GO) annotations related to this gene include sequence-specific DNA binding and protein heterodimerization activity. An important paralog of this gene is HIST1H2BH. |
| Gene ID | 54145 |
| Alternative | H2BFS; Histone H2B type F-S; Histone H2B.s; H2B/s |
| Others | Histone H2B Polyclonal Antibody detects endogenous levels of Histone H2B protein. |
| Accession | P57053 |
| Observed Band(KD) | 14 |
Fig.1. Western Blot analysis of various cells using Histone H2B Polyclonal Antibody diluted at 1:1000.
Fig.2. Western Blot analysis of COS7 cells using Histone H2B Polyclonal Antibody diluted at 1:1000.
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