| Product name | Histone H2B (Acetyl Lys21) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from part region of human Histone H2B (Acetyl Lys21) protein at amino acid sequence of 21 |
| Host | Rabbit |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;ELISA 1:10000-20000 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | Histone H2B type 1-A; HIST1H2BA; Histone H2B, testis; TSH2B.1; hTSH2B1; Testis-specific histone H2B |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 15kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST1H2BA is intronless and encodes a replication-dependent histone that is a testis/sperm-specific member of the histone H2B family. Transcripts from HIST1H2BA contain a palindromic termination element. HIST1H2BA (Histone Cluster 1 H2B Family Member A) is a Protein Coding gene. Among its related pathways are Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 and DNA Double Strand Break Response. |
| Gene ID | 255626 |
| Alternative | Histone H2B type 1-A; HIST1H2BA; Histone H2B, testis; TSH2B.1; hTSH2B1; Testis-specific histone H2B |
| Others | The antibody detects endogenous levels of Histone H2B when Acetyl occurs at Lys21 |
| Accession | Q96A08/P33778/P62807 |
| Observed Band(KD) | 15 |
Fig.1. Western blot analysis of 3T3 Mouse-kidney KB K562 Hela lysate, antibody was diluted at 1:2000. HRP, Goat Anti-Rabbit IgG (Cat #: A21020) secondary antibody was diluted at 1:20000.
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