| Product name | Histone H2A.X (phospho Ser139) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human Histone H2A.X around the phosphorylation site of S139 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat,Hamster |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | H2AFX; H2AX; Histone H2A.x; H2a/x |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 15 19kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. H2AFX encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. |
| Gene ID | 3014 |
| Alternative | H2AFX; H2AX; Histone H2A.x; H2a/x |
| Others | Phospho-Histone H2A.X (S139) Polyclonal Antibody detects endogenous levels of Histone H2A.X protein only when phosphorylated at S139. |
| Accession | P16104 |
| Observed Band(KD) | 15 |
Fig.1. Western Blot analysis of 4T1 (1), VEC (2), 293 (3), diluted at 1:500.
Fig.2. Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue. 1, Histone H2A.X (phospho Ser139) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.3. Immunohistochemical analysis of paraffin-embedded mouse testis tissue. 1, Histone H2A.X (phospho Ser139) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.4. Immunohistochemical analysis of paraffin-embedded rat testis tissue. 1, Histone H2A.X (phospho Ser139) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
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