| Product name | GRP78 BiP Polyclonal Antibody |
| Immunogen | Synthetic peptide from human protein at AA range: 1268-1341 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat,Fish |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC-f:1:50-300;ELISA 1:10000-20000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 78kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The protein encoded by HSPA5 (heat shock protein family A (Hsp70) member 5) is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell. |
| Gene ID | 3309 |
| Alternative | 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP |
| Others | The antibody detects endogenous GRP78 BiP. |
| Accession | P11021 |
| Observed Band(KD) | 78 |
Fig.1. Western blot analysis of 3T3 Cell Lysate, antibody was diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200.
Fig.3. Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200.
Fig.4. Immunohistochemical analysis of paraffin-embedded human-colon-cancer, antibody was diluted at 1:200.
Fig.5. Immunohistochemical analysis of paraffin-embedded human-colon-cancer, antibody was diluted at 1:200.
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