| Product name | Glypican-3 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human Glypican-3 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:20000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | GPC3; OCI5; Glypican-3; GTR2-2; Intestinal protein OCI-5; MXR7 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 70kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. Glypican-3 encoded by GPC3 can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. |
| Gene ID | 2719 |
| Alternative | GPC3; OCI5; Glypican-3; GTR2-2; Intestinal protein OCI-5; MXR7 |
| Others | Glypican-3 Polyclonal Antibody detects endogenous levels of Glypican-3 protein. |
| Accession | P51654 |
| Observed Band(KD) | 70 |
Fig.1. Western Blot analysis of HepG2 cells using Glypican-3 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded rat-liver, antibody was diluted at 1:100.
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