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GADD34 Polyclonal Antibody

GADD34 Polyclonal Antibody

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Specification

Product name GADD34 Polyclonal Antibody
Immunogen Synthesized peptide derived from part region of human GADD34 protein at amino acid sequence of 430-490
Host Rabbit
Reactivity Human,Rat,Mouse,
Applications WB,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;ELISA 1:10000-20000
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Protein phosphatase 1 regulatory subunit 15A; Growth arrest and DNA damage-inducible protein GADD34; Myeloid differentiation primary response protein MyD116 homolog

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 73kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PPP1R15A is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of PPP1R15A by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. PPP1R15A (Protein Phosphatase 1 Regulatory Subunit 15A) is a Protein Coding gene. Diseases associated with PPP1R15A include Malignant Pleural Mesothelioma. Among its related pathways are Protein processing in endoplasmic reticulum and Beta-Adrenergic Signaling.
Gene ID 23645
Alternative Protein phosphatase 1 regulatory subunit 15A; Growth arrest and DNA damage-inducible protein GADD34; Myeloid differentiation primary response protein MyD116 homolog
Others The antibody detects endogenous levels of GADD34
Accession O75807
Observed Band(KD) 73

Image & description

Fig.1. Western blot analysis of Mouse-BRAIN lysate, antibody was diluted at 1:1000. HRP, Goat Anti-Rabbit IgG (Cat #: A21020) secondary antibody was diluted at 1:20000.

Fig.1. Western blot analysis of Mouse-BRAIN lysate, antibody was diluted at 1:1000. HRP, Goat Anti-Rabbit IgG (Cat #: A21020) secondary antibody was diluted at 1:20000.

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