| Product name | Fyn (phospho Tyr530) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human Fyn around the phosphorylation site of Y530 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | FYN; Tyrosine-protein kinase Fyn; Proto-oncogene Syn; Proto-oncogene c-Fyn; Src-like kinase; SLK; p59-Fyn |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 60kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | FYN is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The FYN proto-oncogene, Src family tyrosine kinase associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. |
| Gene ID | 2534 |
| Alternative | FYN; Tyrosine-protein kinase Fyn; Proto-oncogene Syn; Proto-oncogene c-Fyn; Src-like kinase; SLK; p59-Fyn |
| Others | Phospho-Fyn (Y530) Polyclonal Antibody detects endogenous levels of Fyn protein only when phosphorylated at Y530. |
| Accession | P06241 |
| Observed Band(KD) | 60 |
Fig.1. Western Blot analysis of LOVO+UV cells using Phospho-Fyn (Y530) Polyclonal Antibody diluted at 1:2000.
Fig.2. Western blot analysis of 3T3 lysis using Phospho-Fyn (Y530) antibody. Antibody was diluted at 1:2000.
Fig.3. Western blot analysis of 3T3 lysis using Phospho-Fyn (Y530) antibody. Antibody was diluted at 1:2000.
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