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FRS2 Polyclonal Antibody

FRS2 Polyclonal Antibody

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Specification

Product name FRS2 Polyclonal Antibody
Immunogen Synthesized peptide derived from human FRS2 around the non-phosphorylation site of Y196
Host Rabbit
Reactivity Human,Mouse
Applications WB,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;ELISA 1:40000;Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative FRS2; Fibroblast growth factor receptor substrate 2; FGFR substrate 2; FGFR-signaling adaptor SNT; Suc1-associated neurotrophic factor target 1; SNT-1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 65kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background FRS2 is a gene on chromosome 12q15 that encodes an adapter protein which links activated FGR and NGF receptors to downstream signalling pathways. Fibroblast growth factor receptor substrate 2 plays a key role in activating MAP kinases and in phosphorylating PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, in response to ligand-mediated activation of FGFR1. Fibroblast growth factor receptor substrate 2 modulates signalling via SHC1 by competing with it for a common binding site on NTRK1. It is usually part of heteromultimeric protein complexes, and it is highly expressed in the heart, brain, spleen, lungs, liver, skeletal muscle, kidneys and testes.
Gene ID 10818
Alternative FRS2; Fibroblast growth factor receptor substrate 2; FGFR substrate 2; FGFR-signaling adaptor SNT; Suc1-associated neurotrophic factor target 1; SNT-1
Others FRS2 Polyclonal Antibody detects endogenous levels of FRS2 protein.
Accession Q8WU20
Observed Band(KD) 65

Image & description

Fig.1. Western Blot analysis of various cells using FRS2 Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of various cells using FRS2 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of HepG2 cells using FRS2 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of HepG2 cells using FRS2 Polyclonal Antibody diluted at 1:1000.

Fig.3. Western blot analysis of KB lysis using FRS2 antibody. Antibody was diluted at 1:1000.

Fig.3. Western blot analysis of KB lysis using FRS2 antibody. Antibody was diluted at 1:1000.

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