| Product name | Frizzled-7 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the N-terminal region of human Frizzled-7 at AA range: 20-100 |
| Host | Rabbit |
| Reactivity | Human,Mouse |
| Applications | WB,Flow Cyt,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;Flow Cyt 1:50-200;IF/ICC1:100-500;ELISA 1:5000-20000 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | FZD7; Frizzled-7; Fz-7; hFz7; FzE3 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 64kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Members of the frizzled gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. Frizzled-7 contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas. |
| Gene ID | 8324 |
| Alternative | FZD7; Frizzled-7; Fz-7; hFz7; FzE3 |
| Others | Frizzled-7 Polyclonal Antibody detects endogenous levels of Frizzled-7 protein. |
| Accession | O75084 |
| Observed Band(KD) | 64 |
Fig.1. Western Blot analysis of various cells using Frizzled-7 Polyclonal Antibody diluted at 1:2000.
Fig.2. Western Blot analysis of hela cells using Frizzled-7 Polyclonal Antibody diluted at 1:2000.
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