| Product name | FGF-12 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human FGF-12 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:20000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | FGF12; FGF12B; FHF1; Fibroblast growth factor 12; FGF-12; Fibroblast growth factor homologous factor 1; FHF-1; Myocyte-activating factor |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 27kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Fibroblast growth factor 12 encoded by FGF12 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This growth factor lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. The specific function of this gene has not yet been determined. Two alternatively spliced transcript variants encoding distinct isoforms have been reported. |
| Gene ID | 2257 |
| Alternative | FGF12; FGF12B; FHF1; Fibroblast growth factor 12; FGF-12; Fibroblast growth factor homologous factor 1; FHF-1; Myocyte-activating factor |
| Others | FGF-12 Polyclonal Antibody detects endogenous levels of FGF-12 protein. |
| Accession | P61328 |
| Observed Band(KD) | 27 |
Fig.1. Western Blot analysis of 22RV-1, BT474, SHSY5Y cells using FGF-12 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.
Fig.3. Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.
Fig.4. Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.
Fig.5. Immunohistochemical analysis of paraffin-embedded Mouse-brain, antibody was diluted at 1:100.
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