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FANCD2 Polyclonal Antibody

FANCD2 Polyclonal Antibody

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Specification

Product name FANCD2 Polyclonal Antibody
Immunogen Synthesized peptide derived from human FANCD2 around the non-phosphorylation site of S222
Host Rabbit
Reactivity Human,Mouse,Rat
Applications WB,IHC,IF,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative FANCD2; FACD; Fanconi anemia group D2 protein; Protein FACD2

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 166kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. FANCD2 encodes the protein for complementation group D2. Fanconi anemia group D2 protein is monoubiquinated in response to DNA damage, resulting in its localization to nuclear foci with other proteins (BRCA1 AND BRCA2) involved in homology-directed DNA repair. Alternative splicing results in multiple transcript variants.
Gene ID 2177
Alternative FANCD2; FACD; Fanconi anemia group D2 protein; Protein FACD2
Others FANCD2 Polyclonal Antibody detects endogenous levels of FANCD2 protein.
Accession Q9BXW9
Observed Band(KD) 166

Image & description

Fig. Western Blot analysis of various cells using FANCD2 Polyclonal Antibody diluted at 1:500.

Fig. Western Blot analysis of various cells using FANCD2 Polyclonal Antibody diluted at 1:500.

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