| Product name | ERLIN1/2 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from ERLIN1/2 at AA range: 31-80 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;ELISA 1:10000-20000 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 40kD,42kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Erlin-1 and erlin-2 (SPFH1 and SPFH2) are SPFH domain-containing proteins that belong to the prohibitin family. The N-termal domain of erlin proteins contains ER-targeting sequences responsible for their translocation to the endoplasmic reticulum (ER). In the ER, erlin-1 and erlin-2 specifically associate with the detergent resistant lipid raft microdomain of the membrane. Erlin-1 may be involved in dentritic cell activation and erlin-2 has been shown to regulate the ER-associated degradation (ERAD) pathway by interacting with endogenous substrates and resulting in their polyubiquitination and degradation. |
| Others | ERLIN1/2 Polyclonal Antibody detects endogenous levels of ERLIN1/2. |
| Accession | O75477/O94905 |
| Observed Band(KD) | 40+42 |
Fig.1. Western Blot analysis of HEPG2 3T3 Hela cells using ERLIN1/2 Polyclonal Antibody diluted at 1:800. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Western Blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.3. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:200.
Fig.4. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:200.
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