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EpoR (phospho Tyr368) Polyclonal Antibody

EpoR (phospho Tyr368) Polyclonal Antibody

Views(3) Publications(0) Catalog no(ABP54138)
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Specification

Product name EpoR (phospho Tyr368) Polyclonal Antibody
Immunogen Synthesized peptide derived from human EpoR around the phosphorylation site of Y368
Host Rabbit
Reactivity Human,Mouse,Rat
Applications WB,IF,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative EPOR; Erythropoietin receptor; EPO-R

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 55kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background EPOR encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.
Gene ID 2057
Alternative EPOR; Erythropoietin receptor; EPO-R
Others Phospho-EpoR (Y368) Polyclonal Antibody detects endogenous levels of EpoR protein only when phosphorylated at Y368.
Accession P19235
Observed Band(KD) 55

Image & description

Fig. Western Blot analysis of K562 cells using Phospho-EpoR (Y368) Polyclonal Antibody.

Fig. Western Blot analysis of K562 cells using Phospho-EpoR (Y368) Polyclonal Antibody.

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